解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::Analyzing patent data from 1995-2005 show the increasing importance of patenting by small and medium-sized biotech companies. ...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt0606-643
更新日期:2006-06-01 00:00:00
abstract::Insertional mutagenesis represents a major hurdle to gene therapy and necessitates sensitive preclinical genotoxicity assays. Cdkn2a-/- mice are susceptible to a broad range of cancer-triggering genetic lesions. We exploited hematopoietic stem cells from these tumor-prone mice to assess the oncogenicity of prototypica...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt1216
更新日期:2006-06-01 00:00:00
abstract::RNA-interference (RNAi) is a potent mechanism, conserved from plants to humans for specific silencing of genes, which holds promise for functional genomics and gene-targeted therapies. Here we show that bacteria engineered to produce a short hairpin RNA (shRNA) targeting a mammalian gene induce trans-kingdom RNAi in v...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt1211
更新日期:2006-06-01 00:00:00
abstract::As biotechnology advances, the day may soon come for the creation of a self-aware, human-nonhuman chimera. The USPTO has ruled on whether a patent may issue on such an organism, but Congress must still legislate a dividing line between human and non-human patentable subject matter. ...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt0506-517
更新日期:2006-05-01 00:00:00
abstract::Dual-color fluorescence cross-correlation spectroscopy (FCCS) is a promising technique for quantifying protein-protein interactions. In this technique, two different fluorescent labels are excited and detected simultaneously within a common measurement volume. Difficulties in aligning two laser lines and emission cros...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt1207
更新日期:2006-05-01 00:00:00
abstract::Numerous biological processes involve the recognition of a specific pattern of binding sites on a target protein or surface. Although ligands displayed by disordered scaffolds form stochastic rather than specific patterns, theoretical models predict that recognition will occur between patterns that are characterized b...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt1204
更新日期:2006-05-01 00:00:00
abstract::Transcriptional silencing, one of the major impediments to gene therapy in humans, is often accompanied by replication during late S-phase. We report that transcriptional silencing and late replication were prevented by DNA sequences that can initiate DNA replication (replicators). When replicators were included in si...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt1202
更新日期:2006-05-01 00:00:00
abstract::Can new approaches for deriving human stem cells circumnavigate existing patents that dominate embryonic stem cell intellectual property? ...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt0406-411
更新日期:2006-04-01 00:00:00
abstract::The Human Proteome Organization (HUPO) recently completed the first large-scale collaborative study to characterize the human serum and plasma proteomes. The study was carried out in different locations and used diverse methods and instruments to compare and integrate tandem mass spectrometry (MS/MS) data on aliquots ...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt1183
更新日期:2006-03-01 00:00:00
abstract::Little is known about the potential of plastids to accomplish post-translational modifications of foreign proteins. In the present study we generated transplastomic tobacco plants that accumulate the outer surface lipoprotein A (OspA)-the basic constituent of the first generation monovalent human vaccine against Lyme ...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt1170
更新日期:2006-01-01 00:00:00
abstract::New cultivars with very erect leaves, which increase light capture for photosynthesis and nitrogen storage for grain filling, may have increased grain yields. Here we show that the erect leaf phenotype of a rice brassinosteroid-deficient mutant, osdwarf4-1, is associated with enhanced grain yields under conditions of ...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt1173
更新日期:2006-01-01 00:00:00
abstract::The construction of synthetic cell-cell communication networks can improve our quantitative understanding of naturally occurring signaling pathways and enhance our capabilities to engineer coordinated cellular behavior in cell populations. Towards accomplishing these goals in eukaryotes, we developed and analyzed two ...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt1162
更新日期:2005-12-01 00:00:00
abstract::Lactobacillus sakei is a psychotrophic lactic acid bacterium found naturally on fresh meat and fish. This microorganism is widely used in the manufacture of fermented meats and has biotechnological potential in biopreservation and food safety. We have explored the 1,884,661-base-pair (bp) circular chromosome of strain...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt1160
更新日期:2005-12-01 00:00:00
abstract::A human pancreatic beta-cell line that is functionally equivalent to primary beta-cells has not been available. We established a reversibly immortalized human beta-cell clone (NAKT-15) by transfection of primary human beta-cells with a retroviral vector containing simian virus 40 large T-antigen (SV40T) and human telo...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt1145
更新日期:2005-10-01 00:00:00
abstract::Type I polyketide synthase (PKS) genes consist of modules approximately 3-6 kb long, which encode the structures of 2-carbon units in polyketide products. Alteration or replacement of individual PKS modules can lead to the biosynthesis of 'unnatural' natural products but existing techniques for this are time consuming...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt1128
更新日期:2005-09-01 00:00:00
abstract::Ensuring adequate manufacturing capacity for recombinant and monoclonal antibodies is one of the greatest challenges facing the biotech industry. The following factors should be taken into account when designing product development and production strategies. ...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt0905-1054
更新日期:2005-09-01 00:00:00
abstract::The tubular gland of the chicken oviduct is an attractive system for protein expression as large quantities of proteins are deposited in the egg, the production of eggs is easily scalable and good manufacturing practices for therapeutics from eggs have been established. Here we examined the ability of upstream and dow...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt1132
更新日期:2005-09-01 00:00:00
abstract::Forster Resonance Energy Transfer has served as a molecular ruler that reports conformational changes and intramolecular distances of single biomolecules. However, such rulers suffer from low and fluctuating signal intensities, limited observation time due to photobleaching, and an upper distance limit of approximatel...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt1100
更新日期:2005-06-01 00:00:00
abstract::Embryonic stem (ES) cells are a promising source of cardiomyocytes, but clinical application of ES cells has been hindered by the lack of reliable selective differentiation methods. Differentiation into any lineage is partly dependent on the regulatory mechanisms of normal early development. Although several signals, ...
journal_title:Nature biotechnology
pub_type: 信件
doi:10.1038/nbt1093
更新日期:2005-05-01 00:00:00
abstract::We developed a modified flagellar type III secretion apparatus to secrete heterologous polypeptides into the growth medium of Escherichia coli. The secretion was facilitated by fusing the 173-bp untranslated DNA fragment upstream of the gene fliC (encoding flagellin) as well as a transcriptional terminator from fliC, ...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt1077
更新日期:2005-04-01 00:00:00
abstract::Although mass spectrometry has become a powerful tool for the functional analysis of biological systems, complete proteome characterization cannot yet be achieved. Instead, the sheer complexity of living organisms demands fractionation of cellular extracts to enable more targeted analyses. Here, we introduce the conce...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt1076
更新日期:2005-04-01 00:00:00
abstract::Systemic gene delivery into muscle has been a major challenge for muscular dystrophy gene therapy, with capillary blood vessels posing the principle barrier and limiting vector dissemination. Previous efforts to deliver genes into multiple muscles have relied on isolated vessel perfusion or pharmacological interventio...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt1073
更新日期:2005-03-01 00:00:00
abstract::Functional genomics and proteomics are identifying many potential drug targets for novel therapeutic proteins, and both rational and combinatorial protein engineering methods are available for creating drug candidates. A central challenge is the definition of the most appropriate design criteria, which will benefit cr...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt1064
更新日期:2005-02-01 00:00:00
abstract::The discovery of large multiprotein complexes in cells has increased the demand for improved heterologous protein production techniques to study their molecular structure and function. Here we describe MultiBac, a simple and versatile system for generating recombinant baculovirus DNA to express protein complexes compr...
journal_title:Nature biotechnology
pub_type: 信件
doi:10.1038/nbt1036
更新日期:2004-12-01 00:00:00
abstract::Transgenic crops are approved for release in some countries, while many more countries are wrestling with the issue of how to conduct risk assessments. Controls on field trials often include monitoring of horizontal gene transfer (HGT) from crops to surrounding soil microorganisms. Our analysis of antibiotic-resistant...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt1009
更新日期:2004-09-01 00:00:00
abstract::We describe a simple technology used to cure an established metastatic disease. Intradermal injection of plasmid DNA encoding a transcriptionally targeted cytotoxic gene, along with hsp70, not only promoted tissue-specific, inflammatory killing of normal melanocytes, but also induced a CD8(+) T-cell-dependent, antigen...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt1007
更新日期:2004-09-01 00:00:00
abstract::The basics of determining valuation will help you to understand what criteria matter most for investors. ...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:
更新日期:2004-08-01 00:00:00
abstract::The expression patterns of eukaryotic genomes are controlled by their chromatin structure, consisting of nucleosome subunits in which DNA of approximately 146 bp is wrapped around a core of 8 histone molecules. Post-translational histone modifications play an essential role in modifying chromatin structure. Here we ap...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt990
更新日期:2004-08-01 00:00:00
abstract::Recent studies have demonstrated the important enzymatic, structural and regulatory roles of RNA in the cell. Here we present a post-transcriptional regulation system in Escherichia coli that uses RNA to both silence and activate gene expression. We inserted a complementary cis sequence directly upstream of the riboso...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt986
更新日期:2004-07-01 00:00:00
abstract::A problem in analyzing microarray-based gene expression data is the separation of genes causally involved in a disease from innocent bystander genes, whose expression levels have been secondarily altered by primary changes elsewhere. To investigate this issue systematically in the context of a class of complex human d...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt965
更新日期:2004-05-01 00:00:00
abstract::The green fluorescent protein (GFP) from the jellyfish Aequorea victoria and its fluorescent homologs from Anthozoa corals have become invaluable tools for in vivo imaging of cells and tissues. Despite spectral and chromophore diversity, about 100 cloned members of the GFP-like protein family possess common structural...
journal_title:Nature biotechnology
pub_type: 杂志文章,评审
doi:10.1038/nbt943
更新日期:2004-03-01 00:00:00
abstract::Three national patent offices have consulted on patents that cover protein three-dimensional structural data and pharmacophores, with significant implications for the biotechnology industry. ...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt0104-109
更新日期:2004-01-01 00:00:00
abstract::The biological and physical sciences share a common interest in small structures (the definition of 'small' depends on the application, but can range from 1 nm to 1 mm). A vigorous trade across the borders of these areas of science is developing around new materials and tools (largely from the physical sciences) and n...
journal_title:Nature biotechnology
pub_type: 杂志文章,评审
doi:10.1038/nbt872
更新日期:2003-10-01 00:00:00
abstract::Tocotrienols are the primary form of vitamin E in seeds of most monocot plants, including cereals such as rice and wheat. As potent antioxidants, tocotrienols contribute to the nutritive value of cereal grains in human and livestock diets. cDNAs encoding homogentisic acid geranylgeranyl transferase (HGGT), which catal...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt853
更新日期:2003-09-01 00:00:00
abstract::Protein phosphorylation is a dominant mechanism of information transfer in cells, and a major goal of current proteomic efforts is to generate a system-level map describing all the sites of protein phosphorylation. Recent efforts have focused on developing technologies for enriching and quantifying phosphopeptides. Id...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt863
更新日期:2003-09-01 00:00:00
abstract::A fibrinolytic agent consisting of a tissue-type plasminogen activator (tPA) coupled to the surface of red blood cells (RBCs) can dissolve nascent clots from within the clot, in a Trojan horse-like strategy, while having minimal effects on preexisting hemostatic clots or extravascular tissue. After intravenous injecti...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt846
更新日期:2003-08-01 00:00:00
abstract::A T-cell receptor mimic peptide (TCRpep) consisting of an 8-amino-acid peptide, homologous to the transmembrane region of the T-cell receptor (TCR) alpha chain, blocks T-cell activation after systemic application. When dendritic cells (DCs) were transduced to secrete the TCRpep and injected into mice, evidence of immu...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt842
更新日期:2003-08-01 00:00:00
abstract::High-density short oligonucleotide microarrays have become a widely used tool for measuring gene expression on a large scale. However, details of the mechanism of binding on microarrays remain unclear. Short oligonucleotide probes currently synthesized on microarrays are often ineffective as a result of limited sequen...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt836
更新日期:2003-07-01 00:00:00
abstract::We show that transplantation of adult bone marrow-derived cells expressing c-kit reduces hyperglycemia in mice with streptozotocin-induced pancreatic damage. Although quantitative analysis of the pancreas revealed a low frequency of donor insulin-positive cells, these cells were not present at the onset of blood gluco...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt841
更新日期:2003-07-01 00:00:00
abstract::This report describes the selection of highly efficient antibody catalysts by combining chemical selection from a synthetic library with directed in vitro protein evolution. Evolution started from a naive antibody library displayed on phage made from fully synthetic, antibody-encoding genes (the Human Combinatorial An...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt828
更新日期:2003-06-01 00:00:00